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Point blank ph5/2/2023 ![]() ![]() coli ST155 as a phage production host thereby minimising the possibility of accidental release of pathogenic hosts into wastewater. The study establishes the potential of E. enterica augmented wastewater, which resulted in a 3-log reduction in 24 h. As a proof-of-concept, phage ϕPh_SE03 was produced in ST155 by employing a unique Bacteriophage Amplification Reactor-Lytics Broadcasting System and was simultaneously disseminated into S. Lack of antibiotic resistance and absence of functional prophages rendered the host suitable for environmental applications. Survival assay on Caenorhabditis elegans, the absence of Shiga toxin, and enterotoxigenic E. Phages were characterised based on morphology, latent period, burst size, the efficiency of plating, and restriction enzyme profile. coli ST155, the phages demonstrated differential intergeneric infectivity against Salmonella enterica, E. We identified a novel strain, Escherichia coli ST155, that could serve as a production host for three different polyvalent phages (ϕPh_SE03, ϕPh_SD01, and ϕPh_EC01), thus superseding the use of individual isolation hosts. Their production usually requires pathogenic isolation hosts. Bacteriophages are generally specific, and a cocktail of phages is needed to combat different bacterial targets. ![]()
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